Colon lactoflora of rats with alimentary polyhypovitaminosis and modified fat component of diet
The examination was carried out on male Wistar rats with an initial weight 97–121 g. Influence of vitamin provision and composition of fat component in semisynthetic diet on the condition of lactoflora population of intestine were studied. The deficiency of vitamins was caused by fivefold decrease of amount of vitamin mixture added to the feed and by elimination of vitamin E from this mixture. The modification of fat component was made by substitution of sunflower oil for linseed oil in equal amount (the ratio of vegetable oil and animal fat (lard) was 1:1). Duration of the first phase of the experiment was 28 days. Vitamin deficiency in rats, receiving feed with sunflower oil, was accompanied by significant decrease of vitamins A, E, B1 and B2 in the liver, but did not affect the quantity of lactobacilli in caecum content of rats. Enrichment of the diet deficient in vitamins with polyunsaturated omega 3 fatty acids was associated with a statistically significant increase in number of lactobacilli in the intestine compared with the control group (9,78±0,08 opposite 8,82±0,33 lg CFU/g, р=0,018) and group of rats with vitamin deficiency (9,03±0,18 lg CFU/гg р=0,006). On the second stage, replenishment of vitamin deficiency was carried out in the next 14 days by increasing the amount of vitamin mixture to 70 and 200% of vitamin content from a diet in control group. The replenishment has not affected the number of caecum lactobacilli irrespectively of the dose of vitamins and fatty component.
Keywords:alimentary polyhypovitaminosis, polyunsaturated omega-3 fatty acids, linseed oil, caecum, lactobacilli, rats