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Effect of indole-3-carbinol and rutin on rats’ provision by vitamins’ A and E with different fat content in its diet

AbstractEffect of indole-3-carbinol (I-3-C) and rutin (R) supplementation on vitamins A and E status of growing Wistar rats, receiving for 6 or 4 week semi-synthetic diets with different levels (1, 11 and 31%) of fat (lard and sunflower oil at a ratio of 1:1) has been studied. The content of vitamin E was 6, 9 and 15 IU, vitamin A – 400 IU in 100 g of ration. Against the various fat content during the last 7 or 14 days of the experiment rats received respectively I-3-C (20 mg per 1 kg of body weight per day) or R (0,4% of the feed weight). Rat tissues were analyzed for vitamins A (retinol and retinyol palmitate) and E (alpha-tocopherol) by HPLC. Reducing fat content in diet from 11 to 1% was associated with significant (p<0,05) decrease of hepatic retinyl palmitate and alpha-tocopherol (1,6–1,7 times) with constant plasma concentration of retinol and alpha-tocopherol. Raising fat content from 11 to 31% , in contrast, led to increased levels of hepatic retinyl palmitate and alphatocopherol respectively by 13% (p=0,248) and 89% (p=0,006) and plasma ROL of 26% (p=0,024), while the plasma concentration of alpha- tocopherol has not changed. I-3-C and R do not affect the availability of vitamin E in rats, regardless of the fat content in the diet. With excess fat content (31%) in the diet, supplementation of I-3-C and R lowered hepatic RP by 22–52% (p<0,05) compared to rats receiving a diet with adequate fat. Adding of I-3-C to the high-fat diets resulted to a significant reduction of vitamin A concentration in blood plasma by 12% (p=0,024) and in liver by 37% (p=0,002).

Keywords:vitamin A, retinol, retinol palmitate, vitamin E, alpha-tocopherol, vitamins status, indole-3-carbinol, rutin, blood plasma, liver, rats, fat diet

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