Subpopulation content of lymphocytes in peripheral blood in patients with diabetes mellitus type 2 and obesity
AbstractIn the present study was determined the relative quantity subpopulations of lymphocytes, activated T-lymphocytes and CD95-antigen (Fas/APO-1) expression on lymphocytes in the peripheral blood of patients with diabetes mellitus type 2 (DM 2) and obesity in comparison with the healthy persons. The cells were analyzed by the method of flow cytometry using Beckman Coulter FC 500 cytometer. The following of cells subsets: CD19+, CD3+, CD3+CD4+, CD3+CD8+, CD3–CD16+СD56+, CD3+CD16+CD56+, CD3+CD25+, CD3+HLA-DR+, CD45+CD95+ were investigated. In this research was establish the rise of immunoregulatory index (CD3+CD4+/CD3+CD8+) in consequence of decrease the percentages of cytotoxic T-lymphocytes in patients with DM2 and obesity in comparison with the healthy persons. In the peripheral blood of patients with DM2 and obesity were observed a greater level of activated T-lymphocytes (CD3+HLA-DR+), that reflect the increase activity of T-cell immunity. The direct correlation was found between the increased quantity of NKT-cells (CD3+CD16+CD56+) and contain of triglicerides in the peripheral blood of patients with DM2. In these patients also was noted a greater level of lymphocytes expression of CD95-antigen in comparison with the patients with obesity and healthy persons. These results suggest that immune mechanisms play the important role in pathogenesesis of DM2 and obesity.
Keywords:diabetes mellitus type 2, obesity, subpopulations of lymphocytes, markers of activation, cell immunity